Vitamin D, 25-Hydroxy
Testing Information
Sample Type
Serum
Test Codes
630430
CPT Code
82306
Testing Area
Core Lab - Chemistry
Test Methodology
Abbott Alinity - Chemiluminescence Immunoassay
Reference Range
30-100 ng/mL
Expected Turnaround Time
Results Available 24 hours from Receipt
Specimen Collection Criteria
Collect one gold top SST tube (minimum whole blood: 4 mL). Let specimen clot for 30-60 minutes and then centrifuge to separate serum from cells. Refrigerate after centrifugation.
Rejection Criteria
Grossly hemolyzed specimens, quantity not sufficient, grossly lipemic specimens, improperly labeled
Specimen Validity and Retention
Centrifuged SST Tubes:Room Temperature (20-25°C): 4 hoursRefrigerated (2-8°C): 7 daysFrozen (-20°C or greater): UnacceptableSerum Aliquots:Room Temperature (20-25°C): 4 hoursRefrigerated (2-8°C): 7 daysFrozen (-20°C or greater): 7 daysSpecimens retained for 7 days after testing
Courier Transportation
Transport centrifuged tube refrigerated (2-8°C)
Clinical Utility
Vitamin D assays are used to assess for deficiency, which is significant in bone health, hypertension, nephrolithasis, and other disease states.
